Knockdown of <i>GmSOG1</i>Compromises Drought Tolerance in Transgenic Soybean Lines

• 作者: Samuel Aduse Poku Peter Nkachukwu Chukwurah Htut Htet Aung Ikuo Nakamura
• 来源期刊: 美国植物学期刊(英文)
• 学科: 生物学
• 关键词: GmSOG1 DNA Damage Abiotic Stress Drought Stress
• 年，卷（期）: 2021,（1）
• 页码范围: 18-36
• 页数: 19页
• 分类号: Q94

摘要

Plants are regularly exposed to myriads of stress factors that cause tremendous damage to their genetic make-up. To ensure genome stability and survival over several generations under harsher environmental conditions, plants have evolved a unique mechanism for dealing with DNA damage known as the DNA damage response pathway (DDR). It has been proposed that there may exist a relationship between the DNA damage response pathway and abiotic stress response in plants. To further investigate this relationship, we </span><span><span style="font-family:Verdana;">knocked down the soybean suppressor of gamma response 1 gene (</span><i><span style="font-family:Verdana;">GmSOG1</span></i><span style="font-family:Verdana;">),</span></span><span style="font-family:Verdana;"> a master regulatory gene of the DDR, in soybean plants and subjected the generated transgenic plants to drought stress analysis. Gene expression analysis of the </span><i><span style="font-family:Verdana;">GmSOG1</span></i><span style="font-family:Verdana;"> gene in drought stressed soybean tissues revealed high levels of expression in buds and young leaves. The root lengths and root fresh weights of transgenic soybean plants grown on Murashige and Skoog media supplemented with Gamborg B5 vitamins (MSB5 media) containing 200 mM mannitol for 10 days were significantly lesser than those of drought stressed wild-type plants. Polyethylene glycol (PEG) induced drought stress assay </span><i><span style="font-family:Verdana;">in vivo </span></i><span style="font-family:Verdana;">resulted in significant damage in transgenic plants compared with wild-type plants. Also, the relative expressions of known drought responsive transcription factors such as </span><i><span style="font-family:Verdana;">GmDREB1</span></i><span style="font-family:Verdana;"> and </span><i><span style="font-family:Verdana;">GmLEA</span></i><span style="font-family:Verdana;"> as well as antioxidation related genes like </span><i><span style="font-family:Verdana;">GmAPX</span></i><span style="