Effect of New Fly Maggot Protein Feed on mRNA Expression of TOR Signaling Pathway-related Genes in Loaches (Misgurnus anguillicaudatus)

• 作者: Haitao ZHANG Yunlong LI Yongliang CHEN Kailang GU Feng GAO
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: New fly maggot protein feed LOACH TOR signaling pathway
• 年，卷（期）: 2021,（1）
• 页码范围: 9-11
• 页数: 3页
• 分类号: R73

摘要

[Objectives] This study was conducted to explore the effects of new fly maggot protein feed on the mRNA expression of genes related to the TOR signaling pathway in loaches(Misgurnus anguillicaudatus). [Methods] Two kinds of test feed with equal nitrogen and energy were prepared by replacing 60% of the fish meal in the control group with the new fly maggot protein feed, i.e., Diet1(control group) and Diet2(60% fish meal replacement group). The feeding experiment was carried out in an indoor circulating water system, and the breeding period was 60 d. [Results] For the livers, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly higher than those in the Diet1 group(P<0.05), while the expression of 4 EBP2 was lower than in the Diet1 group(P<0.05);and as to the muscles, the mRNA levels of TOR and 4EBP1 in the Diet2 group were significantly lower than those in the Diet1 group(P<0.05), while there was no significant change in the mRNA level of 4EBP2 between the two groups. [Conclusions] The replacement of fish meal by fly maggot cultures affected the mRNA expression of TOR, 4EBP1 and 4EBP2 in loach livers and muscles.