Effects of Feeding OmniGen-AF<sup>&reg</sup>during Superovulation on <i>in Vitro</i>Development of Embryos Recovered from Donor Beef Cows

• 作者: Alexandria P. Snider Derek J. Mclean Alfred R. Menino Jr.
• 来源期刊: 动物科学期刊(英文)
• 学科: 农学
• 关键词: BOVINE OmniGen Follicle Stimulating Hormone Embryo Development
• 年，卷（期）: 2021,（1）
• 页码范围: 37-49
• 页数: 13页
• 分类号: S82

摘要

Embryo quality is crucial when selecting embryos for transfer. Variation in quality may be attributed to poor oocytes, semen, stress, inflammation, and potential immune system dysregulation. OmniGen-AF^&reg (OG) feeding supports immune system function and animal health. Our laboratory recently reported lower percent degenerate embryos recovered and increased plasma progesterone in beef cattle donors fed OG during superovulation. <i></span><i><span style="font-family:Verdana;">In vitro</span></i><span style="font-family:Verdana;"></i> development of embryos recovered from donor cows fed OG prior to collection is presented here. Embryos were recovered from 24 beef cows assigned to four treatment groups: 0 g OG/hd/d and 200 mg Folltropin^&reg-V (FSH) (0/200);0 g OG/hd/d and 400 mg FSH (0/400), 56 g OG/hd/d, 200 mg FSH (56/200) and 56 g OG/hd/d and 400 mg FSH (56/400). Good to excellent quality early blastocysts were cultured for 8 d. and development through hatching, embryonic volume and plasminogen activator (PA) production were quantified. The complete protocol was repeated 90 - 120 d later as Replicate 2. Optimal development was observed by embryos recovered from 0/200 cows where percent blastocysts hatching was greater </span><span style="font-family:Verdana;">(</span><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></i></span><span style="font-family:Verdana;"> < 0.05)</span> <span style="font-family:Verdana;">compared to 56/200 and 0/400 cows and embryonic volume was greatest (</span><span style="font-family:Verdana;"><i></span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></i></span><span style="font-family:Verdana;"> < 0.05) in Replicate 1. However, percent blastocysts hatching from 0/200 cows</span><span style="font-family:Verdana;"> was similar (<i></span><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></i> > 0.10) to 56/400 cows an