Screening the Best Material for Ploidy Identification of Sugarcane-related Genera

• 作者: Lianying MAO Yiyun GUI Jinju WEI Haibi LI Ronghua ZHANG Hui ZHOU Xihui LIU
• 来源期刊: 农业生物技术:英文版
• 学科: 医学
• 关键词: Flow cytometry Chromosome ploidy SUGARCANE Cell suspension
• 年，卷（期）: 2021,（1）
• 页码范围: 12-14
• 页数: 3页
• 分类号: R73

摘要

[Objectives] Flow cytometry is widely used to identify plant chromosome ploidy because of its simplicity, rapidity and accuracy. Chromosome ploidy identification is an important part of sugarcane ploidy breeding and application research. It is particularly important to find out the best detection part for ploidy identification in sugarcane. [Methods] The cell suspensions of sugarcane stem tips and leaves were prepared by blade chopping method. The cell suspensions were detected by flow cytometry. The best position for ploidy identification was determined by comparing the cell suspension prepared from stem tips and cell suspension prepared from leaves. [Results] The results showed that the cell suspension dissociated from stem tips was more clear than that from leaf cell suspension;the proportion of non-adherent cells in the suspension prepared from stem tips was larger than that from the leaf cell suspension;the main peak of the stem tip cell suspension was single and the number of cells was more than that of the stem tip cell suspension by flow cytometry. Using the known ploidy ‘Badilar’ as the internal reference, the ploidy of cyathomi 87-16 was detected to be 8.37. [Conclusions] Sugarcane shoot tips are an ideal material for ploidy identification. This study provides a theoretical basis for selecting the best detection site for ploidy identification of sugarcane.