Evaluation of the Antimicrobial Activity of Endophytic Fungus Isolated from <i>Cocos nucifera</i>(L.) Cotyledon against Medically-Important Pathogens

• 作者: Byran N. Fuego Kyla G. Romano Charlmaine D. Pinlac Gary Antonio C. Lirio
• 来源期刊: 生物科学与医学(英文)
• 学科: 生物学
• 关键词: Endophytic Fungi Antimicrobial Activity Cocos nucifera L. Natural Product Agar Plug Diffusion Medically-Important Pathogens
• 年，卷（期）: 2021,（1）
• 页码范围: 86-97
• 页数: 12页
• 分类号: Q93

摘要

The study aimed to evaluate the endophytic fungi isolated from <em>Cocos nucifera</em> (L.) cotyledon for their possible antimicrobial activity. A total of twelve endophytic fungal species were successfully isolated, from which five were selected and presumptively identified, including <em>Aspergillus niger</em>, <em>Raffaelea sp.</em>, <em>Aspergillus flavus</em>, <em>Cladosporium sp.</em> and <em>Pseudallescheria spp.</em> based on their macro and microscopic characters. The agar blocks of actively growing pure culture were assessed for antibacterial activity against five human pathogenic bacterial and fungal strains: <em>Escherichia coli</em> (BIOTECH 1634), <em>Bacillus subtilis</em> (BIOTECH 1697), <em>Staphylococcus aureus</em> (BIOTECH 1592), <em>Serratia marcescens</em> (BIOTECH 1748), and <em>Candida tropicalis</em> (BIOTECH 2085). Among the selected five endophytic used for antimicrobial activity screening, the <em>Raffaelea sp.</em> (CN-EF 2) has shown a high zone of inhibition of 11 mm against <em>Bacillus subtilis</em>, 18 mm against <em>Escherichia coli</em>, 15 mm against <em>Staphylococcus aureus</em>, and 11 mm against <em>Candida tropicalis</em>. Correspondingly, <em>Aspergillus niger</em> (CN-EF 1) has shown a zone of inhibition of 16 mm against <em>C. tropicalis</em>. The endophytic fungus <em>Raffaelea sp.</em> was effective against all test organisms except for <em>Serratia marcescens</em>, while <em>Aspergillus niger</em> was only effective for pathogenic fungi <em>C. albicans</em>. Hence, further in-depth studies of the isolated endophytic fungi are needed to determine the mechanisms behind the endophytes’ inhibitory effects.