Sex determination is a complex biological process, through which the sex of an organism is established in a binary fate decision [1,2]. There are two main determining mechanisms:1) genotypic sex determination (GSD), whereby the individual's sex is determined by its genotype;and 2) environ-mental sex determination (ESD), where the sex is driven by different external factors, such as temperature, pH, and social interactions [1].
Zebrafish sex determination can be affected by various factors. Recent studies have demonstrated that multiple sexrelated genes interact with each other as a network to dictate the gender, which is known as polygenic sex determination [3].