One-Step Multiplex PCR for Simultaneous Detection and Identification of Eight Medically Important <i>Candida</i>Species

• 作者: Akira Fukatsu Osamu Tsuzukibashi Hidenori Suzuk Katsuhiro Asaka Yoshinori Ono Mana Fuchigami Taira Kobayashi Satoshi Uchibori Yuji Takahashi Chiaki Komine Yoshimi Konishi Yuki Ogura Hiroko Omori Masanobu Wakami Hiroshi Murakami Masahiko Fukumoto
• 来源期刊: 口腔学期刊(英文)
• 学科: 数学
• 关键词: Candida Candida albicans One-Step Multiplex PCR PCR Method
• 年，卷（期）: 2021,（1）
• 页码范围: 14-24
• 页数: 11页
• 分类号: O17

摘要

Recently, the incidence of<span> </span><i><span>Candida</span></i><span> infections has substantially increased. Conventional identification methods for </span><i><span>Candida</span></i><span> species are technically difficult to conduct and cannot accurately distinguish each species. The purpose of the present study was to design primers to identify and detect simultaneously</span><span> </span><span>eight medically important </span><i><span>Candida</span></i><span> species using one-step multiplex PCR. PCR primers were designed based on partial sequences of intergenic spacer (IGS) and internal transcribed spacer (ITS) genes of eight medically important </span><i><span>Candida</span></i><span> species. These primers were able to distinguish each </span><i><span>Candida</span></i><span> species and did not display cross-reactivity with representative </span><i><span>Candida </span></i><span>species other than the eight</span><i><span> Candida</span></i><span> species. Moreover, our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and worked without requiring DNA extraction.</span>