Detection of Campylobacter sp. from Poultry Feces in Ouagadougou, Burkina Faso

• 作者: Assèta Kagambèga Alexandre Thibodeau Daniel K. Soro Nicolas Barro Philippe Fravalo
• 来源期刊: 食品与营养科学(英文)
• 学科: 数学
• 关键词: CAMPYLOBACTER Poultry Feces Selective Method Multiplex PCR
• 年，卷（期）: spyyykxyw,（2）
• 页码范围: 107-114
• 页数: 8页
• 分类号: O17

摘要

<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""> <i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;"> contamination in poultry and poultry product has been reported worldwide. The present study aims to determine the prevalence of </span><i><span style="font-family:Verdana;">Campylobacter</span></i><span style="font-family:Verdana;"> in poultry feces using selective enrichment Bolton broth and multiplex PCR. </span><b><span style="font-family:Verdana;">Method:</span></b><span style="font-family:Verdana;"> Two methods were used in this study</span></span><span style="font-family:Verdana;">:</span><span style="font-family:Verdana;"> the first </span><span style="font-family:Verdana;">was</span><span style="font-family:""> <span style="font-family:Verdana;">direct</span><span style="font-family:Verdana;"> plating of poultry feces into mCCDA agar plates. </span><span style="font-family:Verdana;">The second</span><span style="font-family:Verdana;">, three</span><span style="font-family:Verdana;"> antibiotics were used at different concentrations to add in Bolton broth supplemented. These antibiotics were Rifampicin (Oxoid, </span><span style="font-family:Verdana;">Nepean, Ontario) with 10</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/L, colistin (Oxoid, Nepean, Ontario) with 1</span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/mL and 2</span><span style="font-family:""> </span><span style="font-family:Verdana;">mg/mL;trimethoprim (Oxoid, Nepean, Ontario) with 10</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">mg/L. The colonies with typical </span><i><span style="font-family:Verdana;">campylobacter</span></i><span style="font-family:Verdana;"> morphology on blood agar (little, red </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> ring colonies) were further identified to th