Decoding Cortical Glial Cell Development

• 作者: Xiaosu Li Guoping Liu Lin Yang Zhenmeiyu Li Zhuangzhi Zhang Zhejun Xu Yuqun Cai Heng Du Zihao Su Ziwu Wang Yangyang Duan Haotian Chen Zicong Shang Yan You Qi Zhang Miao He Bin Chen Zhengang Yang
• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: Original Articles
• 页码范围: 440-460
• 页数: 21页
• 语种: 英文

摘要

Mouse cortical radial glial cells (RGCs) are primary neural stem ceils that give rise to cortical oligodendrocytes,astrocytes,and olfactory bulb (OB) GABAergic interneurons in late embryogenesis.There are fundamental gaps in understanding how these diverse cell subtypes are generated.Here,by combining single-cell RNA-Seq with intersectional lineage analyses,we show that beginning at around E16.5,neocortical RGCs start to generate ASCL1+EGFR+apical multipotent intermediate progenitors (MIPCs),which then differentiate into basal MIPCs that express ASCL1,EGFR,OLIG2,and MKI67.These basal MIPCs undergo several rounds of divisions to generate most of the cortical oligodendrocytes and astrocytes and a subpopulation of OB interneurons.Finally,single-cell ATAC-Seq supported our model for the genetic logic underlying the specification and differentiation of cortical glial cells and OB interneurons.Taken together,this work reveals the process of cortical radial glial cell lineage progression and the developmental origins of cortical astrocytes and oligodendrocytes.