Effects of Cryofrequency in the Different Swine Skin Tissue Layers: <i>In Vitro</i>and <i>in Vivo</i>Study

• 作者: Rafaella Rêgo Maia Patrícia Froes Meyer Josebel da Costa Emerenciano Joseela Joyce Barbosa da Silva Júlio Davi Costa e Silva Liliane Santos de Vasconcellos Rodrigo Marcel Valentim da Silva Tarciso Bruno Montenegro Sampaio Ciro Dantas Soares
• 来源期刊: 生物科学与医学(英文)
• 学科: 工学
• 关键词: CRYOTHERAPY Radio Frequency ADIPOSITY COLLAGEN Temperature
• 年，卷（期）: swkxyyxyw_2021,（3）
• 页码范围: 94-104
• 页数: 11页
• 分类号: TN9

摘要

<strong>Introduction:</strong> Cryofrequency is a novel therapy which was developed with the combination of cold (cryotherapy) and heat (radiofrequency) application, causing a thermal shock effect on skin tissue. The thermal shock is believed to alter local metabolism. <strong>Objective:</strong> To investigate the effect of cryofrequency on the skin tissue of a mini pig in an <em>in vitro </em>and <em>in vivo</em> experimental model. <strong>Methodology:</strong> A small swine of the <em>S. domesticus </em>species, a male mini-pig (30 kg weight) was elected for the <em>in vivo</em> procedure subject. The <em>in vitro</em> step was performed on the extracted abdominal part of another subject of the same species and breed. Then, after the application of cryofrequency in the monopolar and bipolar form <em>in vivo</em>, a histological analysis was performed. <strong>Results: </strong>A higher temperature variation was observed comparing the pilot study <em>in vitro </em>with respect to the experimental one <em>in vivo</em>. Microscopically, an increase of collagen fibers and blood supply and decreased adipose tissue was observed after cryofrequency application. <strong>Conclusion:</strong> With the bipolar cryofrequency, no reduction of adipose tissue was observed, but regarding the connective tissue, extensive areas of collagen deposition were observed, as well as a great amount of new-formed collagen fibers.