Tetrahydroxystilbene glucoside protects against sodium azide-induced mitochondrial dysfunction in human neuroblastoma cells

• 作者: Ru-yi Zhang Xu Zhang Lan Zhang Yan-chuan Wu Xue-jing Sun Lin Li
• 来源期刊: 中草药（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: Original Articles
• 页码范围: 255-260
• 页数: 6页
• 语种: 英文

摘要

Objective:Mitochondrial dysfunction is evident in the early stage of Alzheimer's disease (AD).Therefore development of drugs that protect mitochondrial function is a promising strategy for AD.The present work was to investigate the effects of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucosides (TSG) on a mitochondrial dysfunction cell model induced by sodium azide and elucidate the underlying mecha-nisms.Methods:Mitochondrial membrane potential (MMP) was detected by a fluorescence method.Cellular adenosine triphosphate (ATP) level was measured using a firefly luciferase-based kit.Reactive oxygen species (ROS) was detected using dichlorofluorescin diacetate (DCFH-DA).The expression levels of Bcl-2 and Bax were measured by Western blotting assay.Flow cytometry was utilized to measure apoptosis.Results:Pretreatment of TSG (25-200 μmol/L) for 24 h significantly elevated MMP and ATP content,reduced ROS level and Bax/Bcl-2 ratio,and inhibited apoptosis in SH-SY5Y cells exposed to sodium azide.Conclusion:These re.sults suggest that TSG protects SH-SY5Y cells against sodium azide-induced mito-chondrial dysfunction and apoptosis.These findings are helpful to understand the protective effect of TSG on mitochondria,which are involved in the early stage of AD.