Dual?Wavelength Photosensitive Nano?in?Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis

• 作者: Chengcheng Yin Hua Tang Jinglun Zhang Jinyang Wang Kailun Shen Lingling Zhang Miusi Shi Qin Zhao Yin Xiao Yufeng Zhang Zifan Zhao
• 来源期刊: 纳微快报（英文）
• 年，卷（期）: 2021,（2）
• 页码范围: 124-143
• 页数: 20页
• 语种: 英文

摘要

The immune response of a biomaterial determines its osteoinduc-tive effect. Although the mechanisms by which some immune cells promote regeneration have been revealed, the biomaterial-induced immune response is a dynamic process involving multiple cells. Currently, it is challenging to accu-rately regulate the innate and adaptive immune responses to promote osteoin-duction in biomaterials. Herein, we investigated the roles of macrophages and dendritic cells (DCs) during the osteoinduction of biphasic calcium phosphate (BCP) scaffolds. We found that osteoinductive BCP directed M2 macrophage polarization and inhibited DC maturation, resulting in low T cell response and efficient osteogenesis. Accordingly, a dual-targeting nano-in-micro scaffold (BCP loaded with gold nanocage, BCP-GNC) was designed to regulate the immune responses of macrophages and DCs. Through a dual-wavelength photosensitive switch, BCP-GNC releases interleukin-4 in the early stage of osteoinduction to target M2 macrophages and then releases dexamethasone in the later stage to target immature DCs, creating a desirable inflammatory environment for osteo-genesis. This study demonstrates that biomaterials developed to have specific regulatory capacities for immune cells can be used to control the early inflam-matory responses of implanted materials and induce osteogenesis.