Isolation and characterization of a high-efficiency marine diesel oil-degrading bacterium

• 作者: Jin Gao Jie Ming Meng Xu Xinge Fu Liang-Feng Duan Cong-Chao Xu Yu Gao Jian-Liang Xue Xin-Feng Xiao
• 来源期刊: 石油科学（英文版）
• 年，卷（期）: 2021,（2）
• 所属期刊栏目: PETROCHEMISTRY & CHEMICAL ENGINEERING
• 页码范围: 641-653
• 页数: 13页
• 语种: 英文

摘要

In the recent 50 years,marine oil spills had resulted in severe environmental pollution problems worldwide.In this study,12 petroleum-degrading strains named MJ1 to MJ12,which can use diesel oil as the sole carbon source for growth,were isolated from the seawater in Jiaozhou Bay,China.Strain MJ4 has the highest diesel-degrading rate which is up to 26.54％in 5 days with the diesel oil concentration of 10 g/L.According to the BLAST research,16SrRNA sequence of MJ4 showed 99％similarity to Bacillus megaterium strain.Single-factor experiments and response surface methodology were carried out to optimize the environmental factors and their reciprocal action for affecting the diesel oil degradation process of Bacillus sp.MJ4.Results of single-factor experiments revealed that the highest degradation rate was obtained with temperature of 28℃,pH of 8.8,diesel oil concentration of 25 g/L,P/N ratio of 0.56,nitrogen and phosphorus dosage quantity of 0.35 g/L and 0.18 g/L,respectively.A nonlinear regression equation of diesel oil degradation rate and pH,temperature,P/N ratio was obtained.The model predicted the maximum degradation rate of 72.21％with temperature of 28℃,pH of 8.88,P/N ratio of 0.31,respectively.