Fluorescein-based monitoring of RNA N6-methyladenosine at single-nucleotide resolution

• 作者: Xiao-Min Liu Shen Wang Xianqing Gan Shu-Bing Qian Jun Zhou
• 来源期刊: 分子细胞生物学报（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: Letter to the Editor
• 页码范围: 325-328
• 页数: 4页
• 语种: 英文
• DOI: 10.1093/jmcb/mjaa057

摘要

Dear Editor, N6-methyladenosine (m6A) emerges as an abundant chemical modification on RNAs,which enriches in distinct internal regions linked to divergent aspects of RNA fate (Zhao et al.,2017).Transcriptome-wide mapping of m6A sites using high-throughput sequencing enables comparative analysis of cellular m6A dynamics on particular RNAs under both physiological and stress conditions(Dominissini et al.,2012;Meyer et al.,2015),yet is confined to low resolution of coverage and indistinction of adjacent m6A sites.Broad research has focused on developing sensitive and reliable approaches to probe m6A status on indi-vidual transcript (Li et al.,2016).A DNA polymerase identified from Thermus thermophilus (Tth pol) is in favor of in-corporating thymidine opposite unmodi-fied A over m6A (Harcourt et al.,2013).