Glycosylation of N-hydroxy-pipecolic acid equilibrates between systemic acquired resistance response and plant growth

• 作者: Jianghua Cai Adam Jozwiak Lara Holoidovsky Michael M.Meijler Sagit Meir Ilana Rogachev Asaph Aharoni
• 来源期刊: 分子植物（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Research Articles
• 页码范围: 440-455
• 页数: 16页
• 语种: 英文

摘要

N-hydroxy-pipecolic acid(NHP)activates plant systemic acquired resistance(SAR).Enhanced defense re-sponses are typically accompanied by deficiency in plant development and reproduction.Despite of exten-sive studies on SAR induction,the effects of NHP metabolism on plant growth remain largely unclear.In this study,we discovered that NHP glycosylation is a critical factor that fine-tunes the tradeoff between SAR defense and plant growth.We demonstrated that a UDP-glycosyltransferase(UGT76B1)forming NHP glycoside(NHPG)controls the NHP to NHPG ratio.Consistently,the ugt76b1 mutant exhibits enhanced SAR response and an inhibitory effect on plant growth,while UGT76B1 overexpression attenuates SAR response,promotes growth,and delays senescence,indicating that NHP levels are dependent on UGT76B1 function in the course of SAR.Furthermore,our results suggested that,upon pathogen attack,UGT76B1-mediated NHP glycosylation forms a"hand brake"on NHP accumulation by attenuating the pos-itive regulation of NHP biosynthetic pathway genes,highlighting the complexity of SAR-associated net-works.In addition,we showed that UGT76B1-mediated NHP glycosylation in the local site is important for fine-tuning SAR response.Our results implicate that engineering plant immunity through manipulating the NHP/NHPG ratio is a promising method to balance growth and defense response in crops.