Whole-genome sequencing to detect mutations associated with resistance to insecticides and Bt proteins in Spodoptera frugiperda

• 作者: Fang Guan Jianpeng Zhang Huiwen Shen Xingliang Wang Amanda Padovan Tom K.Walsh Wee Tek Tay Karl H.J.Gordon William James Cecilia Czepak Michael Hilary Otim Donald Kachigamba Yidong Wu
• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Special section: Fall armyworm, Spodoptera frugiperda, in China
• 页码范围: 627-638
• 页数: 12页
• 语种: 英文
• DOI: 10.1111/1744-7917.12838

摘要

The fall armyworm(FAW),Spodoptera frugiperda,is a major pest native to the Americas that has recently invaded the Old World.Point mutations in the target-site proteins acetylcholinesterase-1(ace-1),voltage-gated sodium channel(VGSC)and ryan-odine receptor(RyR)have been identified in S.frugiperda as major resistance mecha-nisms to organophosphate,pyrethroid and diamide insecticides respectively.Mutations in the adenosine triphosphate-binding cassette transporter C2 gene(ABCC2)have also been identified to confer resistance to Cry 1F protein.In this study,we applied a whole-genome sequencing(WGS)approach to identify point mutations in the target-site genes in 150 FAW individuals collected from China,Malawi,Uganda and Brazil.This approach re-vealed three amino acid substitutions(A201S,G227A and F290V)of S.frugiperda ace-1,which are known to be associated with organophosphate resistance.The Brazilian popu-lation had all three ace-1 point mutations and the 227A allele(mean frequency = 0.54)was the most common.Populations from China,Malawi and Uganda harbored two of the three ace-1 point mutations(A201S and F290V)with the 290V allele(0.47-0.58)as the dominant allele.Point mutations in VGSC(T929I,L932F and L1014F)and RyR(I4790M and G4946E)were not detected in any of the 150 individuals.A novel 12-bp insertion mutation in exon 15 of the ABCC2 gene was identified in some of the Brazilian individu-als but absent in the invasive populations.Our results not only demonstrate robustness of the WGS-based genomic approach for detection of resistance mutations,but also provide insights for improvement of resistance management tactics in S.frugiperda.