Cryo-EM structure of the human mitochondrial translocase TIM22 complex

• 作者: Liangbo Qi Qiang Wang Zeyuan Guan Yan Wu Cuicui Shen Sixing Hong Jianbo Cao Xing Zhang Chuangye Yan Ping Yin
• 来源期刊: 细胞研究（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: LETTERS TO THE EDITOR
• 页码范围: 369-372
• 页数: 4页
• 语种: 英文

摘要

Dear Editor,Mitochondria are essential organelles in cellular metabolism,homeostasis,and apoptosis.1,2 Most mitochondrial proteins are synthesized as precursors in the cytosol and then imported into mitochondria by specific protein translocase complexes,including the translocase of the outer membrane complex (TOM complex),the carrier translocase of the inner membrane complex (TIM22 complex),the presequence translocase of the inner membrane complex (TIM23 complex),the sorting and assembly machinery(SAM complex),and the mitochondrial import complex (MIM complex).3 The TIM22 complex is responsible for the translocation and insertion of hydrophobic membrane proteins,including mitochondrial carrier proteins and translocase subunits (Tim17,Tim22 and Tim23).3 In humans,TIM22 is a 440-kDa complex comprising at least six components:the hypothetical channel?forming protein Tim22t three small Tim proteins (Tim9,TimlOa and Tim10b),Tim29 and acyiglycerol kinase (AGK).1 Considering the functional importance of mitochondrial protein import,the TIM22 complex has been linked to many diseases.For example,mutations in the TIM22 gene have been reported to cause early-onset mitochondrial myopathy.4 AGK participates in lipid bio-synthesis,and mutations in the AGK gene lead to Sengers syndrome.2 Mutations in the TIMM8A gene (also called DDP1)cause deafness dystonia syndrome.2