LmIntegrinβ-PS is required for wing morphogenesis and development in Locusta migratoria

• 作者: Xiao-Ming Zhao Niu Niu Jia-Peng Yang Wei-Min Liu Jian-Zhen Zhang
• 来源期刊: 中国昆虫科学（英文版）
• 年，卷（期）: 2021,（3）
• 所属期刊栏目: Original articles
• 页码范围: 705-717
• 页数: 13页
• 语种: 英文
• DOI: 10.1111/1744-7917.12801

摘要

Wings are an important flight organ of insects and their morphogenesis de-pends on a series of cell-to-cell and cell-to-extracellular matrix interactions.Integrin as a transmembrane protein receptor mediates cell-to-cell adhesion,cell-to-extracellular ma-trix interactions and signal transduction.In the present study,we characterized an integrin gene that encodes integrinβ-PS protein in Locusta migratoria.LmIntegrinβ-PS is highly expressed in the wing pads and the middle stages of 5th instar nymphs.Immunohisto-chemical analysis revealed that the LmIntegrinβ-PS protein was localized at the cell base of the two layers of wings.After suppression of LmIntegrinβ-PS by RNA interference,the wing pads or wings were unable to form normally,with a blister wing appearance during nymph to nymph transition and nymph to adult transition.We further found that the dor-sal and ventral epidermis of the wings after dsLmIntegrinβ-PS injection were improperly connected and formed huge cavities revealed by hematoxylin and eosin staining.Further-more,the morphology and structure of the wing cuticle was significantly disturbed which affected the stable arrangement and attachments of the wing epidermis.Moreover,the expression of related cell adhesion genes was significantly decreased in LmIntegrinβ-PS-suppressed L.migratoria,suggesting that LmIntegrinβ-PS is required for the morphogen-esis and development of wings during molting by stabilizing cell adhesion and maintaining the cytoskeleton of these cells.