Indirect somatic embryogenesis and regeneration of Fraxinus mandshurica plants via callus tissue

• 作者: Yang Liu Cheng Wei Hao Wang Xiao Ma Hailong Shen Ling Yang
• 来源期刊: 林业研究（英文版）
• 年，卷（期）: 2021,（4）
• 所属期刊栏目: ORIGINAL PAPERS
• 页码范围: 1613-1625
• 页数: 13页
• 语种: 英文

摘要

S omatic embryogenesis of Fraxinus mandshu-rica has the problems of low somatic embryo (SE) yield, unsynchronized SE development, and a high percentage of deformed SEs. We aimed to improve F. mandshurica SE production by synchronizing SE development, improving SE quality, and inducing root formation to obtain com-plete regenerated plants. Cotyledons of immature zygotic embryos of F. mandshurica were induced to form callus and then SEs. The SE induction percentage from explants differed among 32 mother trees, and the one with the high-est SE induction percentage (29.8％) was used for further experiments. The highest callus induction percentage was 94.2％ on ?-strength Murashige and Skoog medium (MS?) supplemented with 0.15 mg·L ?1 naphthalene acetic acid. The highest callus proliferation coefficient (240.5) was obtained on McCown's Woody Plant Medium containing 0.1 mg·L ?16-benzyl adenine and 0.15 mg·L ?12, 4-dichlorophenoxy-acetic acid. The highest number of SEs (1020.5 g ?1 fresh weight) was obtained on MS? medium supplemented with 1 mg·L ?16-benzyladenine. The highest number of cotyledon embryos (397/g fresh weight) was obtained by incubating materials on medium containing 1 mg·L ?1 abscisic acid and then applying a drying treatment. The cotyledon embryos were milky white, uniformly sized (average length 4.7 mm), and 80％ of them were normal. The SE rooting percentage on ?MS medium containing 0.01 mg·L ?1 NAA was 37.5％. Overall, the germination percentage of SEs was 26.4％, and complete regenerated plants were obtained after transplant-ing and acclimation. These results provide more possibilities for the preservation and breeding of F. mandshurica .