Shexiang Tongxin Dropping Pill(麝香通心滴丸) Reduces Coronary Microembolization in Rats via Regulation of Mitochondrial Permeability Transition Pore Opening and AKT-GSK3β Phosphorylation

• 作者: DING Yu ZHU Hou-yong ZHANG Li-zong GAO Bei-bei ZHOU Liang HUANG Jin-yu
• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2021,（7）
• 所属期刊栏目: Original Articles
• 页码范围: 527-533
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s11655-019-3176-6

摘要

Objective:To investigate the protective effects of Shexiang Tongxin Dropping Pill(麝香通心滴丸,STDP) following sodium laurate-induced coronary microembolization (CME) in rats.Methods:Forty rats were divided int04 groups:the control (sham) group,CME group,low-dose STDP pretreatment group (20 mg·kg-1·d-1),and high-dose STDP pretreatment group (40 mg·kg-1·d-1).The rats were intragastric administrated with STDP 2 weeks before operation.Moreover,the histopathological alterations were observed using optical microscopy and transmission electron microscopy.Antioxidant biomarkers were analyzed by enzyme-linked immunosorbent assay.Mitochondrial functions including the mitochondrial permeability transition pore (mPTP) mtDNA copy number were determined and proteins of AKT/GSK3β were analyzed by Western blot.Results:The rats in the CME group showed a significant increase in the fibrinogen-Iike protein 2 expression level and mitochondrial dysfunction and a decrease in the expression level of antioxidant biomarkers (superoxide dismutase and catalase,P＜0.01 for all).In contrast,the rats in the low-and high-dose STDP pretreatment groups showed a significant decrease in coronary microthrombi (P＜0.05);moreover,STDP restored the antioxidant-related protein activities and mitochondrial function,inhibited mPTP opening,decreased AKT-Ser473 phosphorylation,and increased GSK3β-Ser9 phosphorylation (P＜0.05 or P＜0.01).Conclusion:STDP may be useful for treatment of CME,possibly via regulation of mPTP opening and AKr/GSK3β phosphorylation.