VEGFR2 mediated RAS/MAPK signaling pathway to explore the mechanism of Bushen Antai Granule in the treatment of recurrent abortion

• 作者: Wei Chang Wei-Li Li Sha-Sha Lu
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（9）
• 所属期刊栏目: Original article
• 页码范围: 30-36
• 页数: 7页
• 语种: 英文

摘要

Objective: To investigate the effect of Bushen Antai Granule on the mRNA and protein expression of Ras protein / mitogen activated protein kinase mediated by vascular endothelial growth factor receptor 2 with small interference RNA interference. Methods: Method to construct the placenta microvascular endothelial cells, and the preparation of kidney fetus granule drug-containing serum, select the best drug-containing serum concentration, it can be divided into normal group, the serum siRNA - NC normal serum group, drug serum, siRNA normal serum group, siRNA drug serum group, using real-time fluorescent quantitative PCR, Western blotting, immunofluorescence test respectively the RAS/MAPK mRNA and protein expression. Results: Results there was no significant difference in Ras and MAPK mRNA and protein expression between the normal group and the negative control group (P>0.05).The mRNA and protein expressions of Ras and MAPK in the drug serum group were significantly higher than those in the normal serum group (P<0.01).Ras and MAPK mRNA and protein expression were significantly decreased in siRNA1 normal serum group compared with normal serum group (P<0.01).Ras, MAPK mRNA and protein expression in siRNA1 drug serum group were significantly different from that in siRNA1 normal serum group (P<0.01). Conclusion: Conclusion The therapeutic effect of Bushen Antai Granule on recurrent abortion may be realized by upregulation of RAS/MAPK mRNA and protein expression.