Plant-based, adjuvant-free, potent multivalent vaccines for avian influenza virus via Lactococcus surface display

• 作者: Shi-Jian Song Gyeong-Im Shin Jinyong Noh Jiho Lee Deok-Hwan Kim Gyeongryul Ryu Gyeongik Ahn Hyungmin Jeon Hai-Ping Diao Youngmin Park Min Gab Kim Woe-Yeon Kim Young-Jin Kim Eun-Ju Sohn Chang Seon Song Inhwan Hwang
• 来源期刊: 植物学报（英文版）
• 年，卷（期）: 2021,（8）
• 所属期刊栏目: Metabolism and Biochemistry
• 页码范围: 1505-1520
• 页数: 16页
• 语种: 英文

摘要

Influenza epidemics frequently and unpredictably break out all over the world, and seriously affect the breeding industry and human activity. In-activated and live attenuated viruses have been used as protective vaccines but exhibit high risks for biosafety. Subunit vaccines enjoy high bio-safety and specificity but have a few weak points compared to inactivated virus or live attenuated virus vaccines, especially in low immunogenicity. In this study, we developed a new subunit vaccine platform for a potent, adjuvant-free, and multivalent vaccination. The ectodomains of he-magglutinins (HAs) of influenza viruses were ex-pressed in plants as trimers (tHAs) to mimic their native forms. tHAs in plant extracts were directly used without purification for binding to inactivated Lactococcus (iLact) to produce iLact-tHAs, an antigen-carrying bacteria-like particle (BLP). tHAs BLP showed strong immune responses in mice and chickens without adjuvants. Moreover, si-multaneous injection of two different antigens by two different formulas, tHAH5N6 + H9N2 BLP or a combination of tHAH5N6 BLP and tHAH9N2 BLP, led to strong immune responses to both antigens. Based on these results, we propose combinations of plant-based antigen production and BLP-based delivery as a highly potent and cost-effective platform for multivalent vaccination for subunit vaccines.