Distinct Immune Signatures in Peripheral Blood Predict Chemosensitivity in Intrahepatic Cholangiocarcinoma Patients

• 作者: Tong Wu Ying-Cheng Yang Bo Zheng Xue-Bing Shi Wei Li Wen-Cong Ma Shan Wang Zhi-Xuan Li Yan-Jing Zhu Jian-Min Wu Kai-Ting Wang Yan Zhao Rui Wu Cheng-Jun Sui Si-Yun Shen Xuan Wu Lei Chen Zhen-Gang Yuan Hong-Yang Wang
• 来源期刊: 工程（英文）
• 年，卷（期）: 2021,（10）
• 所属期刊栏目: New Technology of Tumor Diagnosis and Treatment-Article
• 页码范围: 1381-1392
• 页数: 12页
• 语种: 英文

摘要

Intrahepatic cholangiocarcinoma(ICC)is the second most common liver cancer.Chemotherapy remains the main therapeutic strategy for advanced ICC patients,but chemosensitivity varies individually.Here,we applied cytometry by time-of-flight(CyTOF)to establish the immune profile of peripheral blood mononuclear cells(PBMCs)on the single-cell level at indicated time points before,during,and after chemotherapy.Multiplex immunofluorescence staining was applied to examine the spatial distribution of certain immune clusters.Tissue microarrays(TMAs)were used for prognostic evaluation.A total of 20 ICC patients treated with gemcitabine(GEM)were enrolled in our study,including eight cases with good response(R)and 12 cases with non-response(NR).Tremendous changes in PBMC composition,including an increased level of CD4/CD8 double-positive T cells(DPT),were observed after chemother-apy.Patients with higher level of CD4+CD45RO+CXCR3+T cells before treatment had a favorable response to chemotherapy.Our study identified a positive correlation between the percentage of T cell subpopu-lations and clinical response after chemotherapy,which suggests that it is practical to predict the poten-tial response before treatment by evaluating the proportions of the cell population in PBMCs.