Effect of Buyang Huanwu Decoction on the proliferation, migration and adhesion of endothelial progenitor cells in rats with acute myocardial infarction

• 作者: Zhi-Hao Wen De-Qing Huang Ming-Jian Huang Chao-Xin Pan Qing-Gao Wang Yi-Qiang Liang Yan Pang Ya-Ping Cao Qian-Yu Li
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（14）
• 所属期刊栏目: Original article
• 页码范围: 1-6
• 页数: 6页
• 语种: 英文

摘要

Objective: To investigate the intervention effect of Buyang Huanwu Decoction on endothelial progenitor cell function (EPCs), and to explore the therapeutic mechanism of Buyang Huanwu Decoction. Methods: This research take 54 rats were divided into blank group, the control group, model group, Chinese medicine, western medicine group and combine traditional Chinese and western medicine group, tonifying Yang also five decoction and atorvastatin calcium for acute myocardial infarction (AMI) rats group intervention, by using the method of density gradient centrifugation separation training each rat endothelial progenitor cells, using tetramethyl azo salt trace enzyme reaction colorimetry, used in the determination of adhesion ability and improved Boyden chamber, the method of analysis and comparison between groups of endothelial progenitor cells proliferation, adhesion and migration. Results: In 7 days, 14 days and 4 weeks, acute myocardial infarction model of rat peripheral blood EPCs count, proliferation, migration and adhesion ability were compared with control group were significantly decline, tonifying Yang also five decoction group, western medicine control group and combine traditional Chinese and western medicine group of peripheral blood EPCs count, proliferation, migration and adhesion ability compared with model group were significantly increased (P < 0.05), and combine traditional Chinese and western medicine group is the traditional Chinese medicine and western medicine group on the improvement of the function of EPCs effect more significantly (P < 0.05). Conclusion: Statins combined with Buyang Huanwu Decoction can better improve the endothelial function of AMI rats than traditional Chinese medicine or western medicine. Further clinical studies on statins may better improve the endothelial function of AMI patients, and provide a new research entry point for improving the long-term prognosis of AMI patients.