Mitochondrial proteome of mouse oocytes and cisplatin-induced shifts in protein profile

• 作者: Na Zhang An-di Sun Si-man Sun Rui Yang Yan-yan Shi Qi-long Wang Xin-yu Li Ji-hong Ma Wei Yue Bing-teng Xie Jie Qiao Mo Li
• 来源期刊: 中国药理学报（英文版）
• 年，卷（期）: 2021,（12）
• 所属期刊栏目: Chemotherapy
• 页码范围: 2144-2154
• 页数: 11页
• 语种: 英文

摘要

Mitochondria are essential organelles that provide energy for mammalian cells and participate in multiple functions,such as signal transduction,cellular differentiation,and regulation of apoptosis.Compared with the mitochondria in somatic cells,oocyte mitochondria have an additional level of importance since they are required for germ cell maturation,dysfunction in which can lead to severe inherited disorders.Thus,a systematic proteomic profile of oocyte mitochondria is urgently needed to support the basic and clinical research,but the acquisition of such a profile has been hindered by the rarity of oocyte samples and technical challenges associated with capturing mitochondrial proteins from live oocytes.Here,in this work,using proximity labeling proteomics,we established a mitochondria-specific ascorbate peroxidase (APEX2) reaction in live GV-stage mouse oocytes and identified a total of 158 proteins in oocyte mitochondria.This proteome includes intrinsic mitochondrial structural and functional components involved in processes associated with "cellular respiration","ATP metabolism","mitochondrial transport",etc.In addition,mitochondrial proteome capture after oocyte exposure to the antitumor chemotherapeutic cisplatin revealed differential changes in the abundance of several oocyte-specific mitochondrial proteins.Our study provides the first description of a mammalian oocyte mitochondrial proteome of which we are aware,and further illustrates the dynamic shifts in protein abundance associated with chemotherapeutic agents.