Mechanisms of Huanglian Jiedu Decoction in treating coronary heart disease and type 2 diabetes mellitus based on network pharmacology

• 作者: Ji-Lin Fan Ting-Ting Zhu Xiao-Ling Tian Zhen-Yu Xue Wen-Qing Ren Jing-Qi Guo Shi-Liang Zhang
• 来源期刊: 海南医科大学学报（英文版）
• 年，卷（期）: 2021,（15）
• 所属期刊栏目: Original article
• 页码范围: 48-55
• 页数: 8页
• 语种: 英文

摘要

Objective: To explore the common mechanism of Huanglian Jiedu Decoction in treating coronary heart disease and type 2 diabetes by network pharmacology. Methods: All chemical components and targets of the four drugs in Huanglian Jiedu Decoction were retrieved through TCMSP, and the genes were standardized through Uniprot database. Acquire disease targets related to coronary heart disease and diabetes in OMIM and GeneCards databases. The network diagram of "drug-component-target-disease" is constructed by using the software of cytopscape 3.7.2, the PPI network diagram of protein interaction is constructed by using STRING database, and the network diagram of "drug-disease" core target is constructed by using the software of cytopscape 3.7.2. DAVID's online database platform was used to analyze GO biological process and KEGG pathway enrichment of common targets of Huanglian Jiedu Decoction in treating coronary heart disease and type 2 diabetes. Results: 103 active ingredients of Huanglian Jiedu Decoction were retrieved, including 140 acting targets, 5342 coronary heart disease targets, 114 diabetes targets, and 14 common intersection targets of drugs and diseases, involving AR, PPARG, TNF, IL6, CCL2, VEGFA, PON1, etc. The GO biological process analysis results in 98 biological processes, 10 cell components and 10 molecular functions. Among them are positive regulation of gene expression, positive regulation of nitric oxide biosynthesis process, Extracellular space, cytokine activity, steroid hormone receptor activity and other biological processes; The enrichment analysis of KEGG pathway yielded 20 signal pathways (P≤0.05). It mainly involves Malaria, cancer in cancer, HIF-1 signaling pathway, TNF signaling pathway, NOD-like receptor signaling pathway, PI3K-Akt signaling pathway, etc. Conclusion: Huanglian Jiedu Decoction "treats different diseases at the same time" coronary heart disease and type 2 diabetes have the characteristics of multiple components, multiple targets and multiple pathways, which provide theoretical basis for Huanglian Jiedu Decoction to treat coronary heart disease and type 2 diabetes in clinic, but the key targets and pathways of Huanglian Jiedu Decoction to treat diseases still need further experimental verification.