The in situ spectral methods for examining redox status of c-type cytochromes in metal-reducing/oxid

• 作者: Xiaobo Luo Yundang Wu Xiaomin Li Dandan Chen Ying Wang Fangbai Li Tongxu Liu
• 学科: 地球科学
• 关键词: c-type cytochrome In situ spectroscopy Intact cells Metal reducing bacteria Fe(Ⅱ) oxidation bacteria
• 年，卷（期）: 2022,（3）
• 页码范围: 544-547
• 页数: 3页
• 语种: 英文
• DOI: 10.1007/s11631-017-0232-9

摘要

The membrane-associated c-type cytochromes (c-Cyts) have been well known as the key enzymes mediating extracellular electron transfer to terminal electron acceptors, resulting in biogeochemical elemental transformation, contaminant degradation, and nutrient cycling. Although c-Cyts-mediated metal reduction or oxidation have been mainly investigated with the purified proteins of metal reducing/oxidizing bacteria, the in vivo behavior of c-Cyts is still unclear, given the difficulty in measuring the proteins of intact cells. Fortunately, the in situ spectroscopy would be ideal for measuring the reaction kinetics of c-Cyts in intact cells under noninvasive physiological conditions. It can also help the establishment of kinetic/thermodynamic models of extracellular electron transfer processes, which are essential to understand the electron transfer mechanisms at the molecular scale. This review briefly summarizes the current advances in spectral methods for examining the c-Cyts in intact cells of dissimilatory metal reducing bacteria and Fe(Ⅱ)-oxidizing bacteria.