Recent advances in isolation,identification,and culture of mammalian spermatogonial stem cells

• 作者: Hua-Ming Xi Yi-Jie Ren Fa Ren Yu Li Tian-Yu Feng Zhi Wang Ye-Qing Du Li-Kun Zhang Jian-Hong Hu
• 来源期刊: 亚洲男性学杂志（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: REVIEW
• 页码范围: 5-14
• 页数: 10页
• 语种: 英文
• DOI: 10.4103/aja.aja_41_21

摘要

Continuous spermatogenesis depends on the self-renewal and differentiation of spermatogonial stem cells(SSCs).SSCs,the only male reproductive stem cells that transmit genetic material to subsequent generations,possess an inherent self-renewal ability,which allows the maintenance of a steady stem cell pool.SSCs eventually differentiate to produce sperm.However,in an in vitro culture system,SSCs can be induced to differentiate into various types of germ cells.Rodent SSCs are well defined,and a culture system has been successfully established for them.In contrast,available information on the biomolecular markers and a culture system for livestock SSCs is limited.This review summarizes the existing knowledge and research progress regarding mammalian SSCs to determine the mammalian spermatogenic process,the biology and niche of SSCs,the isolation and culture systems of SSCs,and the biomolecular markers and identification of SSCs.This information can be used for the effective utilization of SSCs in reproductive technologies for large livestock animals,enhancement of human male fertility,reproductive medicine,and protection of endangered species.