Developmental Origins of Human Cortical Oligodendrocytes and Astrocytes

• 作者: Lin Yang Zhenmeiyu Li Guoping Liu Xiaosu Li Zhengang Yang
• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: Original Articles
• 页码范围: 47-68
• 页数: 22页
• 语种: 英文

摘要

Human cortical radial glial cells are primary neural stem cells that give rise to cortical glutaminergic projection pyramidal neurons,glial cells (oligodendrocytes and astrocytes) and olfactory bulb GABAergic interneu-rons.One of prominent features of the human cortex is enriched with glial cells,but there are major gaps in understanding how these glial cells are generated.Herein,by integrating analysis of published human cortical single-cell RNA-Seq datasets with our immunohistochemistical analyses,we show that around gestational week 18,EGFR-expressing human cortical truncated radial glial cells(tRGs) give rise to basal multipotent intermediate progen-itors (bMIPCs) that express EGFR,ASCL1,OLIG2 and OLIG1.These bMIPCs undergo several rounds of mitosis and generate cortical oligodendrocytes,astrocytes and olfactory bulb interneurons.We also characterized molec-ular features of the cortical tRG.Integration of our findings suggests a general picture of the lineage progression of cortical radial glial cells,a fundamental process of the developing human cerebral cortex.