Effect of Sodium Salicylate on Calcium Currents and Exocytosis in Cochlear Inner Hair Cells:Implications for Tinnitus Generation

• 作者: Ting Fan Meng-Ya Xiang Ruo-Qiao Zhou Wen Li Li-Qin Wang Peng-Fei Guan Geng-Lin Li Yun-Feng Wang Jian Li
• 来源期刊: 神经科学通报（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: Original Articles
• 页码范围: 69-80
• 页数: 12页
• 语种: 英文

摘要

Sodium salicylate is an anti-inflammatory med-ication with a side-effect of tinnitus.Here,we used mouse cochlear cultures to explore the effects of salicylate treatment on cochlear inner hair cells (IHCs).We found that IHCs showed significant damage after exposure to a high concentration of salicylate.Whole-cell patch clamp recordings showed that 1-5 mmol/L salicylate did not affect the exocytosis of IHCs,indicating that IHCs are not involved in tinnitus generation by enhancing their neuronal input.Instead,salicylate induced a larger peak amplitude,a more negative half-activation voltage,and a steeper slope factor of Ca2+ current.Using noise analysis of Ca2+ tail currents and qRT-PCR,we further found that salicylate increased the number of Ca2+ channels along with Cav1.3 expression.All these changes could act synergistically to enhance the Ca2+ influx into IHCs.Inhibition of intracel-lular Ca2+ overload significantly attenuated IHC death after 10 mmol/L salicylate treatment.These results implicate a cellular mechanism for tinnitus generation in the peripheral auditory system.