Total Ginsenoside Extract from Panax ginseng Enhances Neural Stem Cell Proliferation and Neuronal Differentiation by Inactivating GSK-3β

• 作者: LIN Kai-li ZHANG Ji CHUNG Hau-lam WU Xin-yi LIU Bin ZHAO Bo-xin SZE Stephen Cho-wing ZHOU Ping-zheng YUNG Ken Kin-lam ZHANG Shi-qing
• 来源期刊: 中国结合医学杂志（英文版）
• 年，卷（期）: 2022,（3）
• 所属期刊栏目: Original Articles
• 页码范围: 229-235
• 页数: 7页
• 语种: 英文
• DOI: 10.1007/s11655-021-3508-1

摘要

Objective:To study the effects of total ginsenosides(TG)extract from Panax ginseng on neural stem cell(NSC)proliferation and differentiation and their underlying mechanisms.Methods:The migration of NSCs after treatment with various concentrations of TG extract(50,100,or 200 μg/mL)were monitored.The proliferation of NSCs was examined by a combination of cell counting kit-8 and neurosphere assays.NSC differentiation mediated by TG extract was evaluated by Western blotting and immunofluorescence staining to monitor the expression of nestin and microtubule associated protein 2(MAP2).The GSK-3 β/β-catenin pathway in TG-treated NSCs was examined by Western blot assay.The NSCs with constitutively active GSK-3 βmutant were made by adenovirus-mediated gene transfection,then the proliferation and differentiation of NSCs mediated by TG were further verified.Results:TG treatment significantly enhanced NSC migration(P＜0.01 or P＜0.05)and increased the proliferation of NSCs(P＜0.01 or P＜0.05).TG mediation also significantly upregulated MAP2 expression but downregulated nestin expression(P＜0.01 or P＜0.05).TG extract also significantly induced GSK-3 β phosphorylation at Ser9,leading to GSK-3 β inactivation and,consequently,the activation of the GSK-3 β/β-catenin pathway(P＜0.01 or P＜0.05).In addition,constitutive activation of GSK-3 β in NSCs by the transfection of GSK-3 β S9A mutant was found to significantly suppress TG-mediated NSC proliferation and differentiation(P＜0.01 or P＜0.05).Conclusion:TG promoted NSC proliferation and neuronal differentiation by inactivating GSK-3 β.