Advanced technologies for single-cell in situ protein profiling

• 作者: Wen Zhou Xiaoyue Ni Chen Xie Quli Fan Dingbin Liu
• 来源期刊: 中国科学：化学（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: REVIEWS
• 页码范围: 48-67
• 页数: 20页
• 语种: 英文

摘要

Cells are inherently heterogeneous to achieve a diverse spectrum of biological functions.To understand the underlying protein machinery that achieves these fascinating functions,it is important to develop advanced analytical methods that can profile proteins in their native environment,as protein expression,aggregation,degradation,and regulation define both normal phy-siology as well as pathogenicity.Genome and transcriptome sequencing have seen major advances at the single-cell levels,but comprehensive proteomic profiling is still challenging.The conventional proteomic methods,such as enzyme-linked im-munosorbent assay (ELISA),western blot,and protein chips,can characterize biomarkers of interest.Still,these ensemble techniques are unsuitable for single-cell studies.Increasing evidence has shown the significance of in situ,sensitive,quantitative,and multiplexed profiling of biomarkers in single cells for diagnosis and treatment guidance.Here,we review the recent development of advanced imaging and spectroscopy techniques,including mass cytometry,immunofluorescence,and surface-enhanced Raman spectrometry (SERS) for single-cell proteomic imaging.We also provide our view on the challenges and the outlook.