A rice XANTHINE DEHYDROGENASE gene regulates leaf senescence and response to abiotic stresses

• 作者: Jiangmin Xu Chenyang Pan Han Lin Hanfei Ye Sheng Wang Tao Lu Qianyu Chen Kairu Yang Mei Lu Qian Qian Deyong Ren Yuchun Rao
• 来源期刊: 作物学报（英文版）
• 年，卷（期）: 2022,（2）
• 所属期刊栏目: Research Papers
• 页码范围: 310-322
• 页数: 13页
• 语种: 英文

摘要

Xanthine dehydrogenase,a member of the molybdenum enzyme family,participates in purine metabo-lism and catalyzes the generation of ureides from xanthine and hypoxanthine.However,the mechanisms by which xanthine dehydrogenase affects rice growth and development are poorly understood.In the present study,we identified a mutant with early leaf senescence and reduced tillering that we named early senescence and less-tillering 1(esl1).Map-based cloning revealed that ESL1 encodes a xanthine dehy-drogenase,and it was expressed in all tissues.Chlorophyll content was reduced and chloroplast malde-velopment was severe in the esl1 mutant.Mutation of ESL1 led to decreases in allantoin,allantoate,and ABA contents.Further analysis revealed that the accumulation of reactive oxygen species in esl1 resulted in decreased photosynthesis and impaired chloroplast development,along with increased sensitivity to abscisic acid and abiotic stresses.Ttranscriptome analysis showed that the ESL1 mutation altered the expression of genes involved in the photosynthesis process and reactive oxygen species metabolism.Our results suggest that ESL1 is involved in purine metabolism and the induction of leaf senescence.These findings reveal novel molecular mechanisms of ESL1 gene-mediated plant growth and leaf senescence.