PPARγ2 functions as a tumor suppressor in a translational mouse model of human prostate cancer

• 作者: Fu-Lu Dong Dong-Mei Liu Ting-Ting Lu Feng Li Chong Zhang Qun E Yong-Hui Zhang
• 来源期刊: 亚洲男性学杂志（英文版）
• 年，卷（期）: 2022,（1）
• 所属期刊栏目: ORIGINAL ARTICLE
• 页码范围: 90-96
• 页数: 7页
• 语种: 英文
• DOI: 10.4103/aja.aja_51_21

摘要

Peroxisome proliferator-activated receptors y(PPARy)is a master regulator that controls energy metabolism and cell fate.PPARy2,a PPARy isoform,is highly expressed in the normal prostate but expressed at lower levels in prostate cancer tissues.In the present study,PC3 and LNCaP cells were used to examine the benefits of restoring PPARy2 activity.PPARy2 was overexpressed in PC3 and LNCaP cells,and cell proliferation and migration were detected.Hematoxylin and eosin(H&E)staining was used to detect pathological changes.The genes regulated by PPARy2 overexpression were detected by microarray analysis.The restoration of PPARy2 in PC3 and LNCaP cells inhibited cell proliferation and migration.PC3-PPARy2 tissue recombinants showed necrosis in cancerous regions and leukocyte infiltration in the surrounding stroma by H&E staining.We found higher mixed lineage kinase domain-like(MLKL)and lower microtubule-associated protein 1 light chain 3(LC3)expression in cancer tissues compared to controls by immunohistochemistry(IHC)staining.Microarray analysis showed that PPARy2 gain of function in PC3 cells resulted in the reprogramming of lipid-and energy metabolism-associated signaling pathways.These data indicate that PPARy2 exerts a crucial tumor-suppressive effect by triggering necrosis and an inflammatory reaction in human prostate cancer.