红细胞分化去核因子(EDDF)对细胞分化及肿瘤细胞恶性调控及其相关基因克隆的研究

• 作者: 刘丕旭 刘世广 刘友华 周国平 张世馥 张乐英 张庆一 杜权 汪兆琦 王鑫 章正琰 章静波 董茂庆 薛社普 费仁仁 陈克铨 马文丽 马静
• 来源期刊: 中国学术期刊文摘
• 学科: 医学
• 关键词: 红细胞分化去核因子 肿瘤细胞 调控 erythroid differentiation amino acids identification of gene expression related genes northern blot fetal liver
• 年，卷（期）: 1998,（9）
• 页码范围: 1130-1133
• 页数: 4页
• 分类号: R73
• 语种: 中文

摘要

The role of regulation of EDDF on erythroid differentiation and myelomal cell malignancy an well as cloning of its stage related differentiation genes were studied. Through a serious of cybrid- and hybridization experiments between mammalian erythroid cells, and erythroleukemia or non-erythroid myelomal cells, and by using various cloning techniques performed for cDNA related to erythroid terminal differentiation. It resulted that accumulated evidences verified that the mammalian erythroid cells containing some active proteins designated as EDDF which with a peak concentration at the intermediate/late erythroblast stages precided denucleation process. It could function to regulate the activities of genes in different stages of erythroblast terminal differentiation, and demonstrating a cause-effect relationships of the processes of globin gene expression, nuclear eccentric, pyknotic, denucleation, and the inhibition of malignant growth of myelomal and erythroleukemia cells. Cloning and identification of the stage related to be erythroid terminal differentiation genes from human fetal liver and mouse splenic erythroblasts were performed. Four full-length sequences of cDNA which containing reading frame that encoding different numbers of amino acids were successfully cloned and identified. All of them were tested by northern blot to be expressed only in the erythropoietic organs of probably regulates erythroid terminal differentiation. Also, all the four sequences were accepted by the Gen Bank as novel cDNA sequences that without comparable homology. The Gen Bank assigned No. of the four terminal differentiation related genes are respectively as follows: Accession No.:AA514190(759 bp.encoding 73 amino acids);Accession No.:AF040247(1.2 kb, 147 aa);AF040248(500 bp.107 aa);Accession NO. AF060220(505 bp.102aa).