LC-MS analysis of the formed peptides from N-( O, O-diiso-propyl) phosphoryl aspartic acid

• 来源期刊: 中国化学(英文版)
• 年，卷（期）: 2000,（6）
• 所属期刊栏目: Notes
• 页码范围: 932-935
• 页数: 4页
• 语种: 英文

摘要

IC-ESI-MS method was used to analyze the formed di- and ri- peptide in the reaction system of N-(O, O-diisopropyl) phosphoryl aspartic acid and adenosine. Cluster ions of the peptides were given in the ESI-MS. The structures of these small peptides were confirmed by LC-MS-MS analysis. Compared wih the traditional HPLC-UV detection, this method showed good sensitivity and selectivity for peptide in the presence of compounds with strong UV ahsorption, such as nucleoside and nucleotide.