Electrochemical Study on Interaction of Vincristine with Tubulin

• 来源期刊: 中国化学（英文版）
• 学科: 化学
• 年，卷（期）: 2001,（11）
• 页码范围: 1084-1088
• 页数: 5页
• 分类号: O6
• 语种: 英文

摘要

In Tris (0.005 mol/L)-NaCl (0.05 mol/L) buffer solution (pH=7.10), keeping temperature at 37℃, a highly sensitive reduction peak of the antitumor agent was obtained by Iinear sweep voltammetry. The peak potential is - 1. 56 v (vs.SCE). The peak current is proportional to the concentration of vhncristhne over the range of 2.1 x 10-7-4.2 × 10-6 mol/L with the detection limit of 1.0 × 10-7 mol/L. The behavior of the binding of vincristine to tubulin was studied. The results showed that the reaction of tubulin dimer with vincristine formed an electrochemically active complex to be 1:2. Its stability constant is 2.5 × 1014. The reduction process of the complex is irreversible with adsorptive characteristics.