Objective:To verify through animal experiment the validity of chemical cholecystectomy.Mothods:The expermental objects seven healthy juvenile pigs,hardener was infused into the gallbladder,after infusion the sapmles were collected by pathological examination,according to the different duration under anesthestize.Results:The mucous destructive and digestive process remained with one week,the inflammatory reaction in two weeks,the chronic inflammatory reaction compained a great deal of granulation tissue and scar formation occurred in 4th-8th week,10 weeks latter,the inflammatory reaction reduced,and scar tissue formed.Conlusion:Chemical cholecystecomy is safe and reliable in clinic.