Detecting of PPNG by Molecular Biology Technique

• 来源期刊: 中华性传播感染杂志（英文版）
• 学科: 医学
• 关键词: Neisseria gonorrhea PPNG nPCR
• 年，卷（期）: 2002,（2）
• 页码范围: 42-43
• 页数: 2页
• 分类号: R75
• 语种: 英文

摘要

Objectives: To investigate the prevalence ofpenicillinase-producing Neisseria gonorrhoeae (PPNG) inWuxi.Methods: Nested PCR (nPCR) was used to test for thepresence of β-lactamase DNA in urethral or cervical swabsspecimens from 217 patients with gonorrhea. OnenPCR-positive product was randomly selected for DNAsequencing.Results: PPNG was found in 60 of all 217 specimens(27.65%), and in 33.87% (42/124) of male and 19.35%(18/93) of female cases. The nPCR product was identical toa known clinical PPNG strain.Conclusion: The prevalence of PPNG in STD patients inWuxi is alarmingly high, and this must be taken intoconsideration for successful treatment and prevention ofgonorrhea infections.