COMPARISON BETWEEN IN VITRO FERTILIZATION OUTCOME OF TWO MOTILE SPERM SEPARATION METHODS

• 来源期刊: 亚洲男性学杂志(英文版)
• 学科: 医学
• 年，卷（期）: 2002,（4）
• 页码范围: 302
• 页数: 1页
• 分类号: R698.2
• 语种: 中文

摘要

Objective: To evaluate the efficiency of Percoll density gradient and swim-up methods for motile sperm isolation for in vitro fertilization and embryo transfer (IVF-ET) program. Methods: The fertilization rate, cleavage rate, embryo developing status and pregnancy outcome of 362 IVF cycles using sperm obtained by the two methods were studied. Results: There was no significant difference in fertilization rate and cleavage rate between the Percoll and swim-up groups. Although the two groups showed no significant difference in the embryo cell number, the percentage of embryos with<20% debris was significantly higher in the Percoll group (77.6%) than in swim-up group (65.9%). The pregnancy rate and the life birth rate were also significantly higher (P<0.01) in the Percoll group (43.7% and 70.3%, respectively) than in the swim-up group (36.6 % and 60.7 %, respectively). Conclusion: The efficiency of the Percoll density gradient method is superior to the swim-up method in motile sperm separation for the IVF-E