5α-REDUCTASE TYPE 2 GENE EXPRESSION IN HUMAN TESTIS, EPIDIDYMIS AND VAS DEFERENS

• 来源期刊: 亚洲男性学杂志(英文版)
• 学科: 医学
• 年，卷（期）: 2002,（4）
• 页码范围: 306
• 页数: 1页
• 分类号: R346
• 语种: 中文

摘要

To study the expression pattern of 5α-reductase type 2 gene in human male reproductive organs. Methods: The expression level of 5ct-reductase type 2 gene in human testis, epididymis and vas deferens tissues was determined by in situ hybridization using a digoxin-labeled 5α-reductase type 2 cRNA probe. Results: The brown granules of hybridizing signals distributed in the cytoplasm of the Sertoli and Leydig cells of the testis, the principle cells of epididymis and the epithelial cells of vas deferens, but there was no positive signal in the nuclei of these cells. No positive signal was observed in the germ cells, basement of the testis, interstium of the epididymis and basement and the smooth muscle cells of vas deferens. Conclusion: This study confirmed that the 5a-reductase type 2 gene expressed in the Sertoli and Leydig cells of the testis and the principle cells of the epididymis. The expression pattem of the gene in these cells in the human was similar to that in the rat and monkey. The presence o