Senescence-like changes induced by expression of p21Waf1/Cip1 in NIH3T3 cell line

• 来源期刊: 细胞研究(英文版)
• 学科: 生物学
• 关键词: p21waf1/Cip1, senescence, inducible expression, cell cycle arrest.
• 年，卷（期）: 2002,（4）
• 页码范围: 229-233
• 页数: 5页
• 分类号: Q2
• 语种: 中文

摘要

P21Waf1/Cip1 is a potent cyclin-dependent kinase inhibitor. As a downstream mediator of p53, p21Waf1/cip1involves in cell cycle arrest, differentiation and apoptosis. Previous studies in human cells provided evidencefor a link between p21Waf1/cip1 and cellular senescence. While in murine cells, the role of p21Waf1/Cip1is indefinite. We explored this issue using NIH3T3 cells with inducible p21Waf1/cip1 expression. Induc-tion of p21Waf1/Cip1 triggered G1 growth arrest, and NIH3T3-p21 cells exhibited morphologic features,such as enlarged and flattened cellular shape, specific to the senescence phenotype. We also showed thatp21Waf1/Cip1-transduced NIH3T3 cells expressedβ-galactosidase activity at pH 6.0, which is known to bea marker of senescence. Our results suggest that p21Waf1/cipx can also induce senescence-like changes inmurine cells.