Protective Effects of Resveratrol and its Analogues against Free Radical-Induced Oxidative Hemolysis of Red Blood Cells

• 来源期刊: 中国化学(英文版)
• 年，卷（期）: 2002,（11）
• 页码范围: 1313-1318
• 页数: 6页
• 语种: 英文

摘要

The in vitro oxidative hemolysis of human red blood cells ( RBCs) was used as a model to study the free radical-induced damage of biological membranes and the protective effect of resveratroi (3,5,4′-trihydroxy-trans-stilbene, 1) and its analogues, i. e., 4-hydroxy-trans-stilbene(2), 3, 5-dihydroxytrans-stilbene (3), 3,4-dihydroxy-trans-stilbene (4), 4,4′-dihydroxy-trans-stilbene (5) and 2, 4, 4′-trihydroxy-trans-stilbene (6). The hemolysis of RBCs was induced by a water-soluble free radical initiator 2, 2′-azobis ( 2-amidinopropane hydrochloride) (AAPH). It was found that addition of AAPH at 37 ℃ to the suspension of RBCs caused fast hemolysis after a short period of inhibition period, and addition of 1-6 significantly suppressed the hemolysis. Compound 4 which bears an ortho-dihydroxyl functionality showed nuch more effective anti-hemolysis activity than that of resveratrol and the other analogues.