Resonance Scattering Spectral Determination of HAS with a New Scattering Enhanced Reagent of K3[Fe(CN)6]

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2002,（12）
• 页码范围: 1566-1572
• 页数: 7页
• 语种: 英文

摘要

A new resonance scattering spectral (RSS) method for the determination of human serum album (HSA) has been proposed with the resonance scattering enhanced reagent of K3[Fe(CN)6]. In the medium of HCl (2,0× 10-3 mol/L), HSA may combine with [Fe(CN)6]3- by intermolecular forces (mainly by electrostatic force) to form {[Fe (CN)6]3n-HSAm+ }k nanoparticle of the ion-association complexes of HSAm+-[Fe(CN)6]3n-. There is a strongest resonance scattering intensity at 351 nm, owing to the existence of the resonance scattering of the nanoparticle, [Fe(CN)6]3- molecular absorption and the non-distribution of the emission intensity of Xe lamp in the range of 200-1000 nm. In addition, two resonance scattering peaks at 470 and 700 nm were observed. Tne HSA concentration in the range of 0-12 μg/mL is lhear to the resonance scattering intensity at 351 mn. The determination limit of this method is 0.1 μg/mL HSA, about ten-fold lower than that of Coomassie brilliant blue protein assay. This method has been used for the determination of HSA in human serum ami synthetic samples with satisfactory results. The mechanism of enhanced resonance scattering light, the TEM of the particle, the concepts of quasi-elastic absorption and tm-elastic absorption were also discussed.