Microcalorimetric Study of the Biological Effects of Zn2+ on Bacillus thuringiensis Growth

• 来源期刊: 中国化学（英文版）
• 年，卷（期）: 2002,（8）
• 页码范围: 746-752
• 页数: 7页
• 语种: 英文

摘要

A microcalorimetric technique was used to investigate the influence of Zn2 + on the growth metabolism of Bacillus thuringiensis. LKB-2277 Bioaciivity Monitor was employed to obtain the power-iime curves, from which the maximum peak-heat output power(Pmax) in the log phase, the growth rate constants (k),the inhibitory raiios (Ⅰ), the generational time (tG) and the total heat effect (Qtotal) in 23 h for the growth metabolism of Bacillus thuringiensis at 28 ℃ can be evaluated. The results indicate that the concentration of Zn2+ affects its growth obviously. Low concentration (0-50 μg/mL) of Zn2 + promotes the growth of Bacillus thuringiensis while high concentration (50-500 μg/mL) of Zn2 + inhibits its growth. When the concentration reached up to 600 μg/mL, it can not grow at all.