Ni2+-PAA Adsorbent for Purifying 6His-OmpTS Recombinant Protein

• 来源期刊: 中国化学快报(英文版)
• 关键词: Affinity matrix polyacrylic acid chelate nickel ion hexa-histidine tagged
• 年，卷（期）: 2002,（5）
• 页码范围: 436-439
• 页数: 4页
• 语种: 英文

摘要

Functional Ni2+-polyacrylic acid (Ni2+-PAA) adsorbent has been prepared for metal chelate affinity chromatography. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subsequent expression in E. coli resulted in the production of hybrid protein 6His-OmpTS that could be purified by Ni2+-PAA affinity chromatography.