CMV-hFasL转基因小鼠容易被小剂量的链尿菌素诱导至糖尿病

• 作者: 余路阳 林波 章振林 郭礼和
• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: transgenic mice Fas Fas ligand type I diabetes mellitus streptozotocin apoptosis interleukin-1 tumor necrosis factor
• 年，卷（期）: 2003,（12）
• 所属期刊栏目: 研究原著
• 页码范围: 1199-1204
• 页数: 6页
• 分类号: R587
• 语种: 英文

摘要

AIM: To investigate the role of Fas-FasL pathway in the pathogenesis of streptozotocin (STZ)-induced type I diabetes mellitus. METHODS: Low dose injections of STZ were used to induce type I diabetes mellitus in the CMV-hFasL transgenic mice. Blood glucose concentration was measured with Glucotrand Plus blood glucose test strips. Expression of hFasL was detected by RT-PCR and Western blotting. The severity of insulitis was determined by histologicalexamination. Expressions of IL- 1 β and TNF-α mRNA in the pancreas were detected by semi-quantitative RT-PCR analysis. Fas expression in apoptotic RIN-5F cells was also confirmed by RT-PCR in vitro. RESULTS: hFasL was expressed in the islets of CMV-hFasL transgenic mice. The transgenic mice were sensitive to diabetic induction than the control WT mice. IL-1 β and TNF-α expressions in the pancreas of CMV-hFasL transgenic mice were far more than that in WT mice. We also found STZ and IL-1β could both induce higher expression of Fas in RIN-5F. The combining of Fas-FasL could lead to the apoptosis of β cells in the CMV-hFasL transgenic mice. CONCLUSION: Fas-FasL interaction plays a significant role in the pathogenic mechanism of type I diabetes mellitus.