Melatonin attenuates β-amyloid-induced inhibition of neurofilament expression

• 来源期刊: 中国药理学报（英文版）
• 学科: 医学
• 关键词: Alzheimer disease amyloid beta-protein neurofilament proteins melatonin
• 年，卷（期）: 2004,（4）
• 页码范围: 447-451
• 页数: 5页
• 分类号: R9
• 语种: 英文

摘要

AIM: To explore the effect of β-amyloid (Aβ) on metabolism of cytoskeletal protein neurofilament, and search for effective cure to the lesion. METHODS: Wild type murine neuroblastoma N2a (N2awt) and N2a stably transfected with wild type amyloid precursor protein (N2aAPP) were cultured. Sandwich ELISA, immunocytochemistry, and Western blot were used respectively to measure the level of Aβ, the expression and phosphorylation of neurofilament proteins. RESULTS: The immunoreactivity of neurofilament protein was almost abolished in N2aAPP, which beard a significantly higher level of Aβ. Melatonin effectively decreased the level of Aβ, and restored partially the level of phosphorylated and non-phosphorylated neurofilament in N2aAPP. CONCLUSION: Overproduction of Aβ inhibits neurofilament expression, and melatonin attenuates the Aβ-induced lesion in cytoskeletal protein.