Effects of puerarin on number and activity of endothelial progenitor cells from peripheral blood

• 来源期刊: 中国药理学报（英文版）
• 关键词: puerarin isoflavones vascular endothelium stem cells cell differentiation vascular endothelial growth factor confocal microscopy
• 年，卷（期）: 2004,（8）
• 页码范围: 1045-1051
• 页数: 7页
• 语种: 英文

摘要

AIM: To investigate whether puerarin can augment endothelial progenitor cells (EPCs) numbers, promote EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity. METHODS: EPCs were characterized as adherent cells by double staining of DiLDL-uptake and lectin binding under a laser scanning confocal microscope.Expression of KDR, VEGFR-2, and AC133 was detected by flow cytometry. EPCs proliferation, migration and in vitro vasculogenesis were determined with MTT assay, modified Boyden chamber assay, and in vitro vasculogenesis kit, respectively. EPCs adhesive assay was performed by replating those on fibronectin-coated dishes, then adherent cells were counted. RESULTS: Incubation of isolated human MNCs with puerarin 0.1-3 mmol/L increased the number of EPCs, EPC proliferative, migratory, adhesive, and in vitro vasculogenesis capacity in a concentrationand time-dependent manner, which reached maximum at 3 mmol/L, 24 h (approximately 1-fold increase, P＜0.01).CONCLUSION: Puerarin enhanced EPCs functional activity.