Angiotensin Ⅱ stimulates phosphorylation of 4E-binding protein 1 and p70 S6 kinase in cultured vascular smooth muscle cells

• 来源期刊: 中国药理学报（英文版）
• 关键词: angiotensin Ⅱ 4E-binding protein 1 p70 S6 kinase phosphatidylinositol 3-kinase vascular smooth muscle
• 年，卷（期）: 2004,（5）
• 页码范围: 593-596
• 页数: 4页
• 语种: 英文

摘要

AIM: To examine the regulatory effects of angiotensin Ⅱ (Ang Ⅱ) on the phosphorylation of 4E-binding protein 1 (4E-BP1) and p70 S6 kinase in cultured vascular smooth muscle cells (VSMC), and the contribution of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB) signaling pathway in this process. METHODS: VSMC obtained from rat thoracic aortas were cultured. The phosphorylation of 4E-BP1 and p70 S6 kinase was detected by immunoblotting. RESULTS: Ang Ⅱ significantly increased the phosphorylation of 4E-BP1 and p70 S6 kinase,with the peaks occurring at, respectively, 10 min and 30 min, after stimulation with Ang Ⅱ. The stimulatory effect of Ang Ⅱ on 4E-BP1 and p70 S6 kinase phosphorylation was abrogated by Ang Ⅱ type 1 receptor (AT1 receptor)antagonist losartan, and suppressed by PI3K inhibitor LY294002 in a concentration-dependent manner.CONCLUSION: Ang Ⅱ treatment of VSMC induces the phosphorylation of 4E-BP1 and p70 S6 kinase via AT1 receptor, and PI3K signaling pathway is involved in this process.